Talk:Bacterial artificial chromosome
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Shape/more details...
Are the BACs linear or circular? What are the ends like if they are linear?
Assembler01 19:14, 15 March 2007 (UTC)assembler01
Circular; They are maintained and amplified within E.coli cells and thus need to be circular. Mistake in the article: According to a paper by Zimmer and Gibbins (1997), Genomics 42, the maximum length for an insert in a BAC is not 350 kbp but probably above 600 kbp. 137.248.179.1 (talk) 13:21, 23 July 2008 (UTC)
BAC and DNA FISH
BACs are used extensively in biomolecular methods other than just cloning. DNA fluorescence in-situ hybridization (DNA FISH) comes to mind. This article should include this information. —Preceding unsigned comment added by 64.134.66.168 (talk) 00:00, 5 August 2010 (UTC) DGD
History of BACs
I have here a statement (that is, locally noted down from a lecture I once heard) that BACs were originally based on the F-plasmid. Evidently different constructs can be used for a BAC. The main article should mention the history, e. g. when they were first used, first called that way, and so forth - ideally close to the start of the article. It may also be helpful to summarize the difference between BACs, PACs and YACs, also from a historic point of view. Right now we don't have this in the main articl here. 2A02:8388:1641:8380:0:0:0:2 (talk) 17:25, 5 December 2019 (UTC)