Y187
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Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain has been sold commercially by Clontech[1] since at least 2000 and is used as a partner with strain AH109 in mating assays.[2]
Genotype of Y187: MATα, ura3-52, his3-200, ade2-101, trp1-901, leu2-3, 112, gal4Δ, met–, gal80Δ, URA3::GAL1UAS-GAL1TATA-lacZ.[3]
References
- ^ Fromont-Racine, Micheline; Rain, Jean-Christophe; Legrain, Pierre (1997). "Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens". Nature Genetics. 16 (3): 277–282. doi:10.1038/ng0797-277. PMID 9207794. S2CID 32591856.
- ^ Guo, Deyin; Rajamäki, Minna-Liisa; Valkonen, Jari (2008). "Protein–Protein Interactions: The Yeast Two-Hybrid System". Plant Virology Protocols. Methods in Molecular Biology. Vol. 451. pp. 421–439. doi:10.1007/978-1-59745-102-4_29. ISBN 978-1-58829-827-0. PMID 18370272.
- ^ http://www.uni-jena.de/unijenamedia/Downloads/faculties/bio_pharm/ls_genetik/Teaching/Grundpraktikum/Y2Hscript_SS11.pdf[permanent dead link]
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