X-Gluc
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Names | |
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IUPAC name
5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid
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Other names
5-Bromo-4-chloro-3-indolyl-β-D-glucuronide
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Identifiers | |
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3D model (JSmol)
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ChemSpider | |
ECHA InfoCard | 100.132.050 |
PubChem CID
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CompTox Dashboard (EPA)
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Properties | |
C14H13BrClNO7 | |
Molar mass | 422.61 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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X-Gluc is a chemical compound with the molecular formula C14H13BrClNO7. It is used as a reagent to detect β-glucuronidase, an enzyme produced by the E. coli bacterium. It is used to detect E. coli contamination in food, water and the urinary tract. In addition, it is widely used in molecular biology experiments to mark and select the expression of target genes (GUS reporter system).[1]
It is often preferred to other detection methods because it is fast (24-hour testing), relatively accurate ( <1% false negatives and <5% false positives ) and the result is easy to observe and interpret. The reagent is safe to transport and easy to store.
See also
References
- ^ Jefferson, RA; Kavanagh, TA; Bevan, MW (1987). "GUS fusions: Beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants". The EMBO Journal. 6 (13): 3901–7. doi:10.1002/j.1460-2075.1987.tb02730.x. PMC 553867. PMID 3327686.
External links
- X-Gluc lab exercise, Susan J Karcher, Purdue University
- Histochemical localization of GUS reporter activity in plant tissues, Stanislav Vitha, Texas A&M University
Categories:
- Chemical articles with multiple compound IDs
- Multiple chemicals in an infobox that need indexing
- Chemical articles with multiple CAS registry numbers
- Articles without EBI source
- Articles without KEGG source
- Articles without UNII source
- ECHA InfoCard ID from Wikidata
- Articles containing unverified chemical infoboxes
- Chembox image size set
- Articles with short description
- Short description matches Wikidata
- Indoles
- Chloroarenes
- Glucuronide esters
- Bromoarenes
- Reagents