Talk:CRISPR/Archive 2

Archive 1

Archive 2

Proposed split

@Alcmaeonid: Thanks for your thoughtful reply.

In thinking more about this, it might be best to split this article into two: one focused on the bacterial defense mechanism and a second on the gene editing technology. Do you agree? If so, then the question is what to call the daughter articles and what is the primary subject. Per WP:PRIMARYTOPIC, the technology is probably primary since most people coming here are interested the technology. On the other hand, the bacterial defense mechanism proceeded the technology by several billion years. Thoughts? Boghog (talk) 13:55, 12 October 2018 (UTC)

Just to chime in, I strongly support this suggestion (although I have little technical expertise). Not just "most", but 95% of everyone visiting this article is probably here for the gene-editing technique, and the current introduction is likely to leave them confused if they don't already know about the defense mechanism. Ornilnas (talk) 23:45, 16 October 2018 (UTC)

I agree with the split and you should refer to the natural system in the gene editing article. I have published several papers on the natural system. --TransControl (talk) 07:15, 4 November 2018 (UTC)

Hi, Having thought about this, I also think a split is a good idea. Split into CRISPR generally including the biological defence mechanism and structure and CRISPR-cas9 etc gene editing as a separate article. 193.61.207.131 (talk) 13:56, 6 November 2018 (UTC)

Agree . I did that in Wiki-pt. 2600:1700:E590:AF10:70D2:F61E:FE31:267D (talk) 00:51, 19 December 2018 (UTC)

Agree, in three other language versions it has already been split (d:Q17310682), --Ghilt (talk) 20:53, 25 January 2019 (UTC)

 Done Thanks to Helito for performing the split. Boghog (talk) 09:56, 17 February 2019 (UTC)

Which two RNA molecules?

The article states:

". The Cas9 endonuclease is a four-component system that includes two small crRNA molecules and trans-activating CRISPR RNA (tracrRNA).[35][36] Jennifer Doudna and Emmanuelle Charpentier re-engineered the Cas9 endonuclease into a more manageable two-component system by fusing the two RNA molecules into a "single-guide RNA" that, when combined with Cas9, could find and cut the DNA target specified by the guide RNA."

It says "fusing the two RNA molecules". But which two of the three RNAs mentioned? The two crRNAs? What about the tracrRNA?

Marchino61 (talk) 01:29, 23 October 2019 (UTC)

CRISPR Applications

Added an application of CRISPR as a diagnostic tool. Needs more work but I would like someone could review it. 100.17.36.101 (talk) 04:00, 28 October 2019 (UTC)

Rephrase "These systems have created CRISPR gene editing"

"These systems have created CRISPR gene editing" should be rephrased since not "these systems" have created CRISPR gene editing, scientists (Jennifer Doudna most of all) did. Right now it reads as if CRISPR gene editing is a process found in nature. --Felix Tritschler (talk) 02:36, 1 January 2021 (UTC)

Agreed, this doesn't make any sense. I have restored some previous text that makes it clear that the application to gene editing is a human exploitation of the bacterial defensive system. Boghog (talk) 17:04, 3 January 2021 (UTC)

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Saurabh

Hello 103.88.59.242 and @Boghog: Although this is WP:CITESELF it is well cited. This satisfies is allowed within reason. I think we should remedy the problem of duplicate <ref>s and keep it. Invasive Spices (talk) 7 October 2022 (UTC) 18:32, 7 October 2022 (UTC)

I have restored it in the application section dealing with crops. It is an appropriate reference in this section of the article. It was not the most appropriate citation in other parts of the article. Boghog (talk) 19:07, 7 October 2022 (UTC)

That looks good to me. Invasive Spices (talk) 7 October 2022 (UTC)

"CRIPSR-Cas system" over "CRISPR/Cas system"

When mentioning CRIPR-Cas system, there is an inconsistent use of both hypen and slash versions of the term.

As per https://twitter.com/shsternberg/status/956167777919930368?s=20 (click "show replies" for the full story), the hypen version seems to be the consensus.

Additionally, other comments under the main tweet thread highlight that hypen can be more easily associated with "and" and slash more with "or". Because CRISPR-Cas system mostly depends on both components (not CRISPR or Cas, but CRISPR and Cas), hypen version seems to be more logical.

Based on this, suggesting to replace the slash version with the hypen one (to begin with, at least for this article). GravityCore (talk) 15:44, 18 May 2023 (UTC)

I'd say citing a tweet is a bit shaky to establish what common practice is (even if it's coming from someone who worked with Doudna herself). However, you don't need to look farther than the titles of the references cited in this article to see that CRISPR-Cas is by far the preferred variant. By my count CRISPR-Cas is used 72 times versus 20 times for CRISPR/Cas. I'll make the change. ― Synpath 23:28, 26 June 2023 (UTC)

The replacement creates two awkward multihyphenated phrases CRISPR-Cas-mediated gene regulation and small, CRISPR-Cas-associated RNA (scaRNA). The first can be reworded, but the sentence itself is awkward imo, and the second can't really since its explaining the acronym. Just noting. ― Synpath 23:39, 26 June 2023 (UTC)