Protein-coding gene in the species Homo sapiens
Kinetochore protein Nuf2 is a protein that in humans is encoded by the NUF2 gene .[5] [6] [7]
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere . Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation .
The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.[7]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000143228 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026683 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Wigge PA, Kilmartin JV (Mar 2001). "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation" . J Cell Biol . 152 (2): 349–60. doi :10.1083/jcb.152.2.349 . PMC 2199619 PMID 11266451 . ^ Nabetani A, Koujin T, Tsutsumi C, Haraguchi T, Hiraoka Y (Oct 2001). "A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint". Chromosoma . 110 (5): 322–34. doi :10.1007/s004120100153 . PMID 11685532 . S2CID 22443613 . ^ a b "Entrez Gene: NUF2 NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)" .
Further reading
DeLuca JG, Moree B, Hickey JM, et al. (2003). "hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells" . J. Cell Biol . 159 (4): 549–55. doi :10.1083/jcb.200208159 . PMC 2173110 PMID 12438418 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Tien AC, Lin MH, Su LJ, et al. (2004). "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model" . Mol. Cell. Proteomics . 3 (1): 93–104. doi :10.1074/mcp.M300072-MCP200 PMID 14602875 . DeLuca JG, Howell BJ, Canman JC, et al. (2004). "Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores" . Curr. Biol . 13 (23): 2103–9. doi :10.1016/j.cub.2003.10.056 PMID 14654001 . S2CID 16183757 . Bharadwaj R, Qi W, Yu H (2004). "Identification of two novel components of the human NDC80 kinetochore complex" . J. Biol. Chem . 279 (13): 13076–85. doi :10.1074/jbc.M310224200 PMID 14699129 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Joseph J, Liu ST, Jablonski SA, et al. (2004). "The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo" . Curr. Biol . 14 (7): 611–7. Bibcode :2004CBio...14..611J . doi :10.1016/j.cub.2004.03.031 PMID 15062103 . S2CID 13757802 . Stucke VM, Baumann C, Nigg EA (2005). "Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1". Chromosoma . 113 (1): 1–15. doi :10.1007/s00412-004-0288-2 . PMID 15235793 . S2CID 28231914 . Meraldi P, Draviam VM, Sorger PK (2004). "Timing and checkpoints in the regulation of mitotic progression" . Dev. Cell . 7 (1): 45–60. doi :10.1016/j.devcel.2004.06.006 PMID 15239953 . Cheeseman IM, Niessen S, Anderson S, et al. (2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension" . Genes Dev . 18 (18): 2255–68. doi :10.1101/gad.1234104 . PMC 517519 PMID 15371340 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . DeLuca JG, Dong Y, Hergert P, et al. (2005). "Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites" . Mol. Biol. Cell . 16 (2): 519–31. doi :10.1091/mbc.E04-09-0852 . PMC 545888 PMID 15548592 . Ciferri C, De Luca J, Monzani S, et al. (2005). "Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore" . J. Biol. Chem . 280 (32): 29088–95. doi :10.1074/jbc.M504070200 PMID 15961401 . Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle" . Proc. Natl. Acad. Sci. U.S.A . 103 (14): 5391–6. Bibcode :2006PNAS..103.5391N . doi :10.1073/pnas.0507066103 PMC 1459365 PMID 16565220 . Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1" . Nature . 441 (7091): 315–21. Bibcode :2006Natur.441..315G . doi :10.1038/nature04727 PMID 16710414 . Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks" . Cell . 127 (3): 635–48. doi :10.1016/j.cell.2006.09.026 PMID 17081983 . S2CID 7827573 . Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry" . Mol. Syst. Biol . 3 (1): 89. doi :10.1038/msb4100134 . PMC 1847948 PMID 17353931 . Liu D, Ding X, Du J, et al. (2007). "Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment" . J. Biol. Chem . 282 (29): 21415–24. doi :10.1074/jbc.M609026200 PMID 17535814 .
