Protein-coding gene in the species Homo sapiens
Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene .[5] [6] [7]
Function MAP1A and MAP1B are microtubule -associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton . MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B.[7]
MAPLC3A is one of the mammalian homologues of yeast ATG8 , an important marker and effector of autophagy .[8]
Regulation MAP1LC3A is regulated by several post-translational modifications . These include covalent linkage of the C-terminus to phosphatidylethanolamine in autophagic membranes, and phosphorylation by protein kinase A ,[9] interact with sequestosome 1 .[10]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000101460 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000027602 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Mann SS, Hammarback JA (May 1997). "Gene localization and developmental expression of light chain 3: a common subunit of microtubule-associated protein 1A(MAP1A) and MAP1B". J Neurosci Res . 43 (5): 535–44. doi :10.1002/(SICI)1097-4547(19960301)43:5<535::AID-JNR3>3.0.CO;2-J . PMID 8833088 . S2CID 23515321 . ^ Pankiv S, Clausen TH, Lamark T, Brech A, Bruun JA, Outzen H, Overvatn A, Bjorkoy G, Johansen T (Aug 2007). "p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy" . J Biol Chem . 282 (33): 24131–45. doi :10.1074/jbc.M702824200 PMID 17580304 . ^ a b "Entrez Gene: MAP1LC3A microtubule-associated protein 1 light chain 3 alpha" .^ Valente G, Morani F, Nicotra G, Fusco N, Peracchio C, Titone R, Alabiso O, Arisio R, Katsaros D, Benedetto C, Isidoro C (2014). "Expression and clinical significance of the autophagy proteins BECLIN 1 and LC3 in ovarian cancer" . Biomed Res Int . 2014 : 462658. doi :10.1155/2014/462658 PMC 4127242 PMID 25136588 . ^ Cherra SJ, Kulich SM, Uechi G, Balasubramani M, Mountzouris J, Day BW, Chu CT (August 2010). "Regulation of the autophagy protein LC3 by phosphorylation" . J. Cell Biol . 190 (4): 533–9. doi :10.1083/jcb.201002108 . PMC 2928022 PMID 20713600 . ^ Shvets E, Fass E, Scherz-Shouval R, Elazar Z (August 2008). "The N-terminus and Phe52 residue of LC3 recruit p62/SQSTM1 into autophagosomes" . J. Cell Sci . 121 (Pt 16): 2685–95. doi :10.1242/jcs.026005 PMID 18653543 .
Further reading
Snásel J, Pichová I (1997). "The cleavage of host cell proteins by HIV-1 protease". Folia Biol. (Praha) . 42 (5): 227–30. doi :10.1007/BF02818986 . PMID 8997639 . S2CID 7617882 . Wallin M, Deinum J, Goobar L, Danielson UH (1990). "Proteolytic cleavage of microtubule-associated proteins by retroviral proteinases" . J. Gen. Virol . 71 (9): 1985–91. doi :10.1099/0022-1317-71-9-1985 PMID 2212989 . Schoenfeld TA, McKerracher L, Obar R, Vallee RB (1989). "MAP 1A and MAP 1B are structurally related microtubule associated proteins with distinct developmental patterns in the CNS" . J. Neurosci . 9 (5): 1712–30. doi :10.1523/JNEUROSCI.09-05-01712.1989 PMC 6569839 PMID 2470876 . Mann SS, Hammarback JA (1994). "Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B" . J. Biol. Chem . 269 (15): 11492–7. doi :10.1016/S0021-9258(19)78150-2 PMID 7908909 . Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination" . Genome Res . 10 (11): 1788–95. doi :10.1101/gr.143000 . PMC 310948 PMID 11076863 . Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs" . Genome Res . 11 (3): 422–35. doi :10.1101/gr.GR1547R . PMC 311072 PMID 11230166 . Bonnet C, Boucher D, Lazereg S, et al. (2001). "Differential binding regulation of microtubule-associated proteins MAP1A, MAP1B, and MAP2 by tubulin polyglutamylation" . J. Biol. Chem . 276 (16): 12839–48. doi :10.1074/jbc.M011380200 PMID 11278895 . Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20" . Nature . 414 (6866): 865–71. Bibcode :2001Natur.414..865D . doi :10.1038/414865a PMID 11780052 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . He H, Dang Y, Dai F, et al. (2003). "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B" . J. Biol. Chem . 278 (31): 29278–87. doi :10.1074/jbc.M303800200 PMID 12740394 . Tanida I, Sou YS, Ezaki J, et al. (2004). "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates" . J. Biol. Chem . 279 (35): 36268–76. doi :10.1074/jbc.M401461200 PMID 15187094 . Kouno T, Mizuguchi M, Tanida I, et al. (2005). "1H, 13C, and 15N resonance assignments of human microtubule-associated protein light chain-3". J. Biomol. NMR . 29 (3): 415–6. doi :10.1023/B:JNMR.0000032505.99071.ea . PMID 15213446 . S2CID 23561733 . Goehler H, Lalowski M, Stelzl U, et al. (2004). "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease" . Mol. Cell . 15 (6): 853–65. doi :10.1016/j.molcel.2004.09.016 PMID 15383276 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline" . Genome Res . 14 (10B): 2136–44. doi :10.1101/gr.2576704 . PMC 528930 PMID 15489336 . Kouno T, Mizuguchi M, Tanida I, et al. (2005). "Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains" . J. Biol. Chem . 280 (26): 24610–7. doi :10.1074/jbc.M413565200 PMID 15857831 . Sou YS, Tanida I, Komatsu M, et al. (2006). "Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16" . J. Biol. Chem . 281 (6): 3017–24. doi :10.1074/jbc.M505888200 PMID 16303767 . Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006" . Nucleic Acids Res . 34 (Database issue): D415–8. doi :10.1093/nar/gkj139 . PMC 1347501 PMID 16381901 .
