Indo-1
Jump to navigation
Jump to search
Names | |
---|---|
IUPAC name
2-[4-(bis(carboxymethyl)amino)-3-[2-[2-(bis(carboxymethyl)amino)-
5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid
| |
Other names
Indo-1
| |
Identifiers | |
3D model (JSmol)
|
|
ChEBI | |
ChemSpider | |
PubChem CID
|
|
UNII | |
CompTox Dashboard (EPA)
|
|
| |
| |
Properties | |
C32H31N3O12 | |
Molar mass | 649.60 g/mol |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
|
Indo-1 is a popular calcium indicator similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emissions peak. The main emission peak in calcium-free solution is 475 nm while in the presence of calcium the emission is shifted to 400 nm. It is widely used in flow cytometry. The penta potassium salt is commercially available and preferred to the free acid because of its higher solubility in water. While Indo-1 is not cell permeable the penta acetoxymethyl ester Indo-1 AM enters the cell where it is cleaved by intracellular esterases to Indo-1. The synthesis and properties of Indo-1 were presented in 1985 by the group of Roger Y Tsien.[1]
References
- ^ Grynkiewicz G, Poenie M, Tsien RY (1985). "A new generation of Ca2+ indicators with greatly improved fluorescence properties". J. Biol. Chem. 260 (6): 3440–50. doi:10.1016/S0021-9258(19)83641-4. PMID 3838314.
External links
Categories:
- Articles with short description
- Short description is different from Wikidata
- Articles without KEGG source
- Articles containing unverified chemical infoboxes
- Chembox image size set
- Short description matches Wikidata
- Biochemistry methods
- Cell imaging
- Chelating agents
- Fluorescent dyes
- Glycol ethers
- Indoles
- All stub articles
- Biochemistry stubs