Protein-coding gene in the species Homo sapiens
Giantin or Golgin subfamily B member 1 is a protein that in humans is encoded by the GOLGB1 gene .[5] [6] [7] Golgi apparatus and is part of the Golgi matrix that is responsible for membrane trafficking in secretory pathway of proteins. This function is key for proper localisation of proteins at the plasma membrane and outside the cell (extracellular region) which is important for cell function that is dependent on for example receptors and the extracellular matrix function. Recent animal model knockout studies of GOLGB1 in mice ,[8] rat ,[9] zebrafish [10] tumoral calcinosis -like phenotype was observed, and in humans such phenotype has been linked to defective glycosyltransferase function (e.g. GALNT3 protein).[11]
Function and Interactions Giantin is a disulfide-linked homodimer which contains several (around 37) coiled-coiled domains. GOLGB1 protein has been shown to interact with ACBD3 and with PLK3 [12] [13] GM130 [14] primary cilia [15] [16] glycosyltransferase expression and calcineurin signalling in tissue culture cells.[17] [18]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000173230 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000034243 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Linstedt AD, Hauri HP (Nov 1993). "Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa" . Mol Biol Cell . 4 (7): 679–93. doi :10.1091/mbc.4.7.679 . PMC 300978 PMID 7691276 . ^ Oka T, Ungar D, Hughson FM, Krieger M (Apr 2004). "The COG and COPI complexes interact to control the abundance of GEARs, a subset of Golgi integral membrane proteins" . Mol Biol Cell . 15 (5): 2423–35. doi :10.1091/mbc.E03-09-0699 . PMC 404034 PMID 15004235 . ^ "Entrez Gene: GOLGB1 golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1" .^ Lan, Yu; Zhang, Nian; Liu, Han; Xu, Jingyue; Jiang, Rulang (2016-07-01). "Golgb1 regulates protein glycosylation and is crucial for mammalian palate development" . Development . 143 (13): 2344–2355. doi :10.1242/dev.134577 . ISSN 0950-1991 . PMC 4958322 PMID 27226319 . ^ Katayama, Kentaro; Sasaki, Tetsu; Goto, Syo; Ogasawara, Kei; Maru, Hiromi; Suzuki, Katsushi; Suzuki, Hiroetsu (November 2011). "Insertional mutation in the Golgb1 gene is associated with osteochondrodysplasia and systemic edema in the OCD rat" . Bone . 49 (5): 1027–1036. doi :10.1016/j.bone.2011.08.001 . PMID 21851869 . ^ Bergen, Dylan J. M.; Stevenson, Nicola L.; Skinner, Roderick E. H.; Stephens, David J.; Hammond, Christina L. (2017-08-15). "The Golgi matrix protein giantin is required for normal cilia function in zebrafish" . Biology Open . 6 (8): 1180–1189. doi :10.1242/bio.025502 . ISSN 2046-6390 . PMC 5576078 PMID 28546340 . ^ Stevenson, Nicola L.; Bergen, Dylan J. M.; Skinner, Roderick E. H.; Kague, Erika; Martin-Silverstone, Elizabeth; Robson Brown, Kate A.; Hammond, Chrissy L.; Stephens, David J. (2017-12-15). "Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression" . Journal of Cell Science . 130 (24): 4132–4143. doi :10.1242/jcs.212308 . ISSN 0021-9533 . PMC 5769581 PMID 29093022 . ^ Sohda, M; Misumi Y; Yamamoto A; Yano A; Nakamura N; Ikehara Y (Nov 2001). "Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin" . J. Biol. Chem . 276 (48): 45298–306. doi :10.1074/jbc.M108961200 ISSN 0021-9258 . PMID 11590181 . ^ Rosing, Mechthild; Ossendorf, Edith; Rak, Alexey; Barnekow, Angelika (July 2007). "Giantin interacts with both the small GTPase Rab6 and Rab1" . Experimental Cell Research . 313 (11): 2318–2325. doi :10.1016/j.yexcr.2007.03.031 . PMID 17475246 . ^ Brandon, Elizabeth; Gao, Yasheng; Garcia-Mata, Rafael; Alvarez, Cecilia; Sztul, Elizabeth (August 2003). "Membrane targeting of p115 phosphorylation mutants and their effects on Golgi integrity and secretory traffic" . European Journal of Cell Biology . 82 (8): 411–420. doi :10.1078/0171-9335-00327 . PMID 14533739 . ^ Bergen, Dylan J. M.; Stevenson, Nicola L.; Skinner, Roderick E. H.; Stephens, David J.; Hammond, Christina L. (2017-08-15). "The Golgi matrix protein giantin is required for normal cilia function in zebrafish" . Biology Open . 6 (8): 1180–1189. doi :10.1242/bio.025502 . ISSN 2046-6390 . PMC 5576078 PMID 28546340 . ^ Asante, D.; MacCarthy-Morrogh, L.; Townley, A. K.; Weiss, M. A.; Katayama, K.; Palmer, K. J.; Suzuki, H.; Westlake, C. J.; Stephens, D. J. (2013-11-15). "A role for the Golgi matrix protein giantin in ciliogenesis through control of the localization of dynein-2" . Journal of Cell Science . 126 (22): 5189–5197. doi :10.1242/jcs.131664 . ISSN 0021-9533 . PMC 3828591 PMID 24046448 . ^ Stevenson, Nicola L.; Bergen, Dylan J. M.; Skinner, Roderick E. H.; Kague, Erika; Martin-Silverstone, Elizabeth; Robson Brown, Kate A.; Hammond, Chrissy L.; Stephens, David J. (2017-12-15). "Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression" . Journal of Cell Science . 130 (24): 4132–4143. doi :10.1242/jcs.212308 . ISSN 0021-9533 . PMC 5769581 PMID 29093022 . ^ Stevenson, Nicola L.; Bergen, Dylan J. M.; Xu, Amadeus; Wyatt, Emily; Henry, Freya; McCaughey, Janine; Vuolo, Laura; Hammond, Chrissy L.; Stephens, David J. (2018-05-01). "Regulator of calcineurin-2 is a centriolar protein with a role in cilia length control" . Journal of Cell Science . 131 (9): jcs212258. doi :10.1242/jcs.212258 . ISSN 0021-9533 . PMC 5992583 PMID 29643119 .
Further reading
Sohda M, Misumi Y, Fujiwara T, et al. (1995). "Molecular cloning and sequence analysis of a human 372-kDA protein localized in the Golgi complex". Biochem. Biophys. Res. Commun . 205 (2): 1399–408. doi :10.1006/bbrc.1994.2821 . PMID 7802676 . Seelig HP, Schranz P, Schröter H, et al. (1994). "Macrogolgin--a new 376 kD Golgi complex outer membrane protein as target of antibodies in patients with rheumatic diseases and HIV infections". J. Autoimmun . 7 (1): 67–91. doi :10.1006/jaut.1994.1006 . PMID 8198703 . Sönnichsen B, Lowe M, Levine T, et al. (1998). "A role for giantin in docking COPI vesicles to Golgi membranes" . J. Cell Biol . 140 (5): 1013–21. doi :10.1083/jcb.140.5.1013 . PMC 2132694 PMID 9490716 . Linstedt AD, Jesch SA, Mehta A, et al. (2000). "Binding relationships of membrane tethering components. The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus" . J. Biol. Chem . 275 (14): 10196–201. doi :10.1074/jbc.275.14.10196 PMID 10744704 . Dirac-Svejstrup AB, Shorter J, Waters MG, Warren G (2000). "Phosphorylation of the vesicle-tethering protein p115 by a casein kinase II-like enzyme is required for Golgi reassembly from isolated mitotic fragments" . J. Cell Biol . 150 (3): 475–88. doi :10.1083/jcb.150.3.475 . PMC 2175190 PMID 10931861 . Alvarez C, Garcia-Mata R, Hauri HP, Sztul E (2001). "The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic" . J. Biol. Chem . 276 (4): 2693–700. doi :10.1074/jbc.M007957200 PMID 11035033 . Sohda M, Misumi Y, Yamamoto A, et al. (2002). "Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin" . J. Biol. Chem . 276 (48): 45298–306. doi :10.1074/jbc.M108961200 PMID 11590181 . Shorter J, Beard MB, Seemann J, et al. (2002). "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115" . J. Cell Biol . 157 (1): 45–62. doi :10.1083/jcb.200112127 . PMC 2173270 PMID 11927603 . Gillingham AK, Pfeifer AC, Munro S (2003). "CASP, the alternatively spliced product of the gene encoding the CCAAT-displacement protein transcription factor, is a Golgi membrane protein related to giantin" . Mol. Biol. Cell . 13 (11): 3761–74. doi :10.1091/mbc.E02-06-0349 . PMC 133590 PMID 12429822 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Anderson NL, Polanski M, Pieper R, et al. (2004). "The human plasma proteome: a nonredundant list developed by combination of four separate sources" . Mol. Cell. Proteomics . 3 (4): 311–26. doi :10.1074/mcp.M300127-MCP200 PMID 14718574 . Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway" . Genome Res . 14 (7): 1324–32. doi :10.1101/gr.2334104 . PMC 442148 PMID 15231748 . Breuza L, Halbeisen R, Jenö P, et al. (2004). "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46" . J. Biol. Chem . 279 (45): 47242–53. doi :10.1074/jbc.M406644200 PMID 15308636 . Malsam J, Satoh A, Pelletier L, Warren G (2005). "Golgin tethers define subpopulations of COPI vesicles". Science . 307 (5712): 1095–8. Bibcode :2005Sci...307.1095M . doi :10.1126/science.1108061 . PMID 15718469 . S2CID 12601850 . Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell . 122 (6): 957–68. doi :10.1016/j.cell.2005.08.029 . hdl :11858/00-001M-0000-0010-8592-0 PMID 16169070 . S2CID 8235923 . Rosing M, Ossendorf E, Rak A, Barnekow A (2007). "Giantin interacts with both the small GTPase Rab6 and Rab1". Exp. Cell Res . 313 (11): 2318–25. doi :10.1016/j.yexcr.2007.03.031 . PMID 17475246 . 
