File:Thermal Proteome Profiling.png

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Summary

Description
English: A cellular lysate is split into control (vehicle) and treatment (compound) groups. Each group is split into aliquots, and each aliquot is heated to a different temperature. As the sample temperature increases, proteins denature and precipitate out of solution. When a test compound is added, target proteins are protected from denaturation by ligand-induced stabilization. After heating, soluble proteins in the supernatant are quantified. Quantification of proteins allows for the generation of protein melting curves. When melting curves shift to the right, a protein is thought to be stabilized by the treatment compound.
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Source BioRender.com
Author BioRender.com

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Captions

An example thermal proteome profiling workflow.

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inception

6 December 2021

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image/png

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current17:31, 6 December 2021Thumbnail for version as of 17:31, 6 December 20214,058 × 4,972 (1.02 MB)commons>Sgold626Uploaded a work by BioRender.com from BioRender.com with UploadWizard

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