File:PMC4848600 viruses-08-00106-g001.png

License

Attribution 4.0 International (CC BY 4.0)

Summary

Author:Zivcec M, Scholte FE, Spiropoulou CF, Spengler JR, Bergeron É ,Viral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention (Openi/National Library of Medicine) Source:https://openi.nlm.nih.gov/detailedresult?img=PMC4848600_viruses-08-00106-g001&query=Crimean%E2%80%93Congo%20hemorrhagic%20fever&it=xg&req=4&npos=8Description:viruses-08-00106-f001: Crimean-Congo hemorrhagic fever virus (CCHFV) virion and replication cycle. The CCHFV virion contains a tri-segmented, negative-sense, single-stranded RNA (vRNA) genome encapsidated by the nucleoprotein (NP) and the RNA-dependent RNA polymerase (RdRp; L protein). Together, vRNA, NP, and RdRp form the genomic ribonucleoprotein complexes (RNP) inside a cellular membrane-derived envelope coated with the mature glycoproteins Gn and Gc. CCHFV attaches to an unidentified cellular receptor and enters the cells in a clathrin-dependent manner. After fusion with the cellular membrane, the viral genomic segments are uncoated and transcribed by L protein into viral mRNA that gain host cell-derived 5′ caps by cap snatching. The viral mRNAs are translated into the NP and L proteins by cytoplasmic ribosomes, while the glycoprotein precursor (GPC) appears to be translated by endoplasmic reticulum (ER)-associated ribosomes. A portion of the newly synthesized NP and L protein are used to replicate the genomic RNA by forming an RNP containing antigenomic RNA (cRNA). The GPC undergoes processing and maturation in the ER and the Golgi, and yields the Gn and Gc. Upon the accumulation of nascent mature glycoproteins and genomic RNPs, new CCHFV particles assembly is believed to occur in the Golgi followed by virion release in Golgi-derived vesicles.