File:PMC4004635 cmr-24-286-g002 (1).png

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Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)

Summary

Author:Nitta K, Kashima T, Mayuzumi H, Akiyama H, Miyanaga T, Hirato J, Kishi S,Department of Ophthalmology, Gunma University School of Medicine,Clinical Department of Pathology, Gunma University Hospital(Open/National Library of Medicine) Source:https://openi.nlm.nih.gov/detailedresult?img=PMC4004635_cmr-24-286-g002&query=Animal-type%20melanoma&it=xg&req=4&npos=4 Description:F2: (a) Macroscopy of the extracted orbital tumor: highly pigmented 6×2 cm large tumor, which ruptured during the extirpation. (b) Hematoxylin and eosin staining of the orbital tumor (×20): melanin-containing cells (melanocytes and melanophages) are abundant in the tumor. (c) Hematoxylin and eosin staining of the orbital tumor after using a bleaching method for melanins (×20): diffuse proliferation of the epithelioid cells and spindle cells, and low-grade nuclear atypia were seen. Small nucleoli were seen in some areas of the tissue. (d) HMB (Human Melanoma Black)-45 staining (×20): positive for atypical cells. (e) Melan-A staining (×20): positive for atypical cells. (f) Hematoxylin and eosin staining of the orbital tumor after using a bleaching method for melanins (×20): vast areas of necrosis were seen.