File:PMC3118360 1743-422X-8-261-1.png

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PMC3118360_1743-422X-8-261-1.png ‎(512 × 276 pixels, file size: 79 KB, MIME type: image/png)

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Attribution 2.0 Generic (CC BY 2.0)

Summary

Author:Barros MC, Galasso TG, Chaib AJ, Degallier N, Nagata T, Ribeiro BM,Cell Biology Department, University of Brasília (Openi/National Library of Medicine) Source:https://openi.nlm.nih.gov/detailedresult?img=PMC3118360_1743-422X-8-261-1&query=yellow%20fever&it=xg&req=4&npos=6 Description:F1: Amplification of the envelope gene (env). Amplification of the Yellow Fever env gene by RT-PCR. (A) Agarose gel (0.8%) eletrophoresis showing the RT-PCR amplification product (lane 2) using the oligonucleotides YFE2460R e YFE878F. Lane 1 shows the molecular mass marker, 1 Kb plus DNA ladder (Invitrogen). (B) Diagram of the Yellow Fever genome (Genebank accession # YFU17066 ) showing the main ORF (polyprotein ORF) and the position of the structural (Cap, pM and E) and non-structural (NS1, NS2a, NS2b, NS3, NS4a and NS5) proteins. The position of the oligonucleotides YFE2460R e YFE878F, used for the amplification reaction are also shown.

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Date/TimeThumbnailDimensionsUserComment
current23:10, 14 October 2021Thumbnail for version as of 23:10, 14 October 2021512 × 276 (79 KB)Ozzie10aaaaAuthor:Barros MC, Galasso TG, Chaib AJ, Degallier N, Nagata T, Ribeiro BM,Cell Biology Department, University of Brasília (Openi/National Library of Medicine) Source:https://openi.nlm.nih.gov/detailedresult?img=PMC3118360_1743-422X-8-261-1&query=yellow%20fever&it=xg&req=4&npos=6 Description:F1: Amplification of the envelope gene (env). Amplification of the Yellow Fever env gene by RT-PCR. (A) Agarose gel (0.8%) eletrophoresis showing the RT-PCR amplification product (lane 2) using the oligonu...

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