File:PMC1142341 1742-4690-2-30-1.png

English: F1: HTLV-3 is closely related to STLV-3. Unrooted phylogenetic tree generated with the Neighbor-joining method, performed in the PAUP program (v4.0b10), on a 180 bp fragment of the tax gene using all full length PTLV-1/2 available sequences and all published STLV-3 tax sequences. The PTLV-1/2/3 strains, including (in bold), the novel sequence generated in this work (Pyl43), were aligned with the DAMBE program (version 4.2.13). The final alignment was submitted to the Modeltest program (version 3.6) to select, according to the Akaike Information Criterion (AIC), the best model to apply to phylogenetic analyses. The selected model was the TrN+G. Bootstrap support (1,000 replicates) is noted on the branches of the tree. The branch lengths are drawn to scale, with the bar indicating 0.1 nucleotide replacement per site.