User talk:Crandel5425/sandbox

Aminoallyl Nucleotide

Lead section: Aminoallyl nucleotides are modified versions of nucleotides(NTPs) used for labeling DNA. These nucleotides, for example, termed aa-dUTP,(u-uracil)are more effective for high density labeling of DNA than pre-labeling. Aminoallyl NTPs can be used for indirect DNA labeling in PCR, nick translation, primer extensions and cDNA synthesis. These labeled NTPs are helpful due to their application in molecular biology labs where radioactive labeling is not allowed or is banned. The different nucleotides; dUTP, dATP, dCTP, DGTP and DTTP (create subunits for these nucleotides ??)Mishasubz (talk) 08:56, 10 March 2014 (UTC)[reply]

Use/Functionality

cDNA (describe each in detail)

PCR nick-translation

random-primed labeling

primer extension.

DNA microarray, which measures the level of gene expression

Post vs. pre-labeling

What are the benefits and disadvantages of using post-synthesis labeling over pre-synthesis labeling like cyanine (cy) labeling

nucleotides coupling with other molecular biological techniques

Use in assay development

Mishasubz (talk) 08:56, 10 March 2014 (UTC)[reply]

Hey Misha, I'm moving this into my user talk page. Thanks for starting this! Crandel5425 (talk) 01:26, 12 March 2014 (UTC)[reply]

unit 8 notes/comments/ideas

Hi Randel,

Should we mention a protocol on labeling with aaNTPs? if so, what do you think about this one

http://www.pangloss.com/seidel/Protocols/amino-allylRT.html Mishasubz (talk) 06:59, 24 March 2014 (UTC)[reply]