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Polymerase chain reaction
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v
t
e
Polymerase chain reaction
techniques
Procedure
Initialization
Cycle
Denaturation
Annealing
Elongation
Final elongation
Final hold
Polymerase
Klenow
T4
and
T7
Taq
Tth
Pfu
Vent
Pwo
Tli
Tfu
Optimization
and
variants
Quantitative polymerase chain reaction
(qPCR)
Reverse transcription polymerase chain reaction
(RT-PCR)
Inverse polymerase chain reaction
(inverse PCR)
Nested polymerase chain reaction
(nested PCR)
Touchdown polymerase chain reaction
Hot start PCR
Overlap extension polymerase chain reaction
(OE-PCR)
Multiplex polymerase chain reaction
(multiplex PCR)
Multiplex ligation-dependent probe amplification
(MLPA)
Co-amplification at lower denaturation temperature PCR
(COLD-PCR)
Random amplification of polymorphic DNA
(RAPD)
History
and people
Kary Mullis
Kjell Kleppe
Alice Chien
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