Talk:Inosine

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Molecular Biology: MCB

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Untitled

if the picture is wrong why isnt it deleted

Why do you think it is wrong?

Base pairing of inosine?

Does inosine really base-pair to guanine? It seems highly unlikely that it could, compared to cytosine, adenine, and uracil. I'm unsure though.

Can anyone confirm this reference to see if it is right? http://www.web-books.com/MoBio/Free/Ch5C4.htm

Molecular Biology of the Cell 4th edition (Alberts et al.) confirms it (fig 6-53) I pairs to U,C,A in bacteria and to U,C in eukaryotes--131.111.8.99 13:00, 24 May 2006 (UTC)[reply]

Inosine does no base pair with guanine, but with cytosine and it is READ or TRANSLATED as guanine.

Note: Inosine is a RNA Nucleoside. Not a DNA Nucleoside. —Preceding unsigned comment added by 4.238.245.42 (talk) 02:12, 9 December 2009 (UTC)[reply]

primers

Is the technique described in the section biotechnology for a specific technique?? because degenerate primers use a mix (a primer with inosine or anything different is expensive as hell, I think they have to stop the machine and add the phosphoramidite manually) if it is not specific but the common mistake it needs correcting: Degenerate bases are not nucleic acid analogues. Some articles use inosine as a test, but not for SNP kits, I think, if there is not a kit that uses inosine, it should be removed.

Liu H. and Nichols,R. (1994) PCR amplification using deoxyinosine to replace entire codon and at ambiguous positions. Biotechniques, 16:, 24–26. [PubMed]. 4.Kamaya H., Sakaguchi,T., Murata,N., Fujimuro,M., Miura,H., Ishikawa,K., Shimizu,M., Inoue,H., Nishimura,S., Matsukage,A., Masutani,C., Hanaoka,F. and Ohtsuka,E. (1992) In vitro replication study of modified bases in RAS sequences. Chem. Pharm. Bull., 40:, 2792–2795

2: Tanaka K, Okamoto A, Saito I. Clear distinction of pyrimidine bases on the complementary strand by fluorescence change of novel fluorescent nucleosides.Nucleic Acids Res Suppl. 2003;(3):171-2. PMID: 14510435

3: Okamoto A, Tanaka K, Fukuta T, Saito I. Design of base-discriminating fluorescent nucleoside and its application to t/c SNP typing. J Am Chem Soc. 2003 Aug 6;125(31):9296-7. PMID: 12889950

Talk:Inosine

Untitled

Base pairing of inosine?

primers

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