40S ribosomal protein S25

RPS25
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4UG0, 4V6X, 5A2Q, 5AJ0, 5FLX
Identifiers
Aliases	RPS25, S25, ribosomal protein S25
External IDs	OMIM: 180465 MGI: 1922867 HomoloGene: 133893 GeneCards: RPS25
Gene location (Human) Chr. Chromosome 11 (human) Band 11q23.3 Start 119,015,712 bp End 119,018,691 bp
Gene location (Mouse) Chr. Chromosome 9 (mouse) Band 9|9 A5.2 Start 44,318,436 bp End 44,321,724 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon pituitary gland anterior pituitary right uterine tube gastric mucosa thyroid gland left lobe of thyroid gland right lobe of thyroid gland canal of the cervix lymph node Top expressed in morula yolk sac lip superior frontal gyrus spermatocyte spermatid Paneth cell renal corpuscle thymus endocardial cushion More reference expression data BioGPS n/a
Gene ontology Molecular function protein binding RNA binding structural constituent of ribosome Cellular component cytosol ribosome cytosolic small ribosomal subunit nucleolus small ribosomal subunit extracellular exosome nucleus nucleoplasm extracellular matrix postsynaptic density Biological process viral transcription SRP-dependent cotranslational protein targeting to membrane translational initiation nuclear-transcribed mRNA catabolic process, nonsense-mediated decay rRNA processing protein biosynthesis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6230 75617 Ensembl ENSG00000118181 ENSMUSG00000009927 UniProt P62851 P62852 RefSeq (mRNA) NM_001028 NM_024266 RefSeq (protein) NP_001019 NP_077228 Location (UCSC) Chr 11: 119.02 – 119.02 Mb Chr 9: 44.32 – 44.32 Mb PubMed search
Wikidata
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40S ribosomal protein S25 (eS25) is a protein that in humans is encoded by the RPS25 gene.

Ribosomes are cellular macromolecules that catalyze protein synthesis across all kingdoms of life. The eukaryotic ribosome consists of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 distinct proteins. The RPS25 gene encodes the eukaryote-specific ribosomal protein eS25 that is a component of the 40S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Interactions

Besides the interactions that position eS25 at its location in the E site region of the 40S ribosomal subunit, eS25 has been suggested to interact with other cellular proteins. One study has found that eS25 interacts with MDM2 as part of a regulatory feedback loop that stabilizes p53. Additionally, eS25 has been shown to interact with CDC5L.

Function

As a ribosomal protein, eS25 likely plays a role in general protein synthesis; however, the RPS25 gene is non-essential for cellular viability in budding yeast and in select mammalian cell lines, implying that it is not essential for eukaryotic protein synthesis. Studies have implicated eS25 in the control of several specialized forms of translation, including that mediated by viral IRESs.

See also

• Eukaryotic translation
• 40S ribosomal subunit
• IRES

Further reading

• Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochemistry and Cell Biology. 73 (11–12): 933–47. doi:10.1139/o95-101. PMID 8722009.
• Li M, Center MS (February 1992). "Regulation of ribosomal protein S25 in HL60 cells isolated for resistance to adriamycin". FEBS Letters. 298 (2–3): 142–4. doi:10.1016/0014-5793(92)80041-E. PMID 1544436. S2CID 9001148.
• Marion MJ, Marion C (May 1988). "Ribosomal proteins S2, S6, S10, S14, S15 and S25 are localized on the surface of mammalian 40 S subunits and stabilize their conformation. A study with immobilized trypsin". FEBS Letters. 232 (2): 281–5. doi:10.1016/0014-5793(88)80753-1. PMID 3378620. S2CID 44333810.
• Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, et al. (December 1994). "Construction of a human full-length cDNA bank". Gene. 150 (2): 243–50. doi:10.1016/0378-1119(94)90433-2. PMID 7821789.
• Imai T, Sudo K, Miwa T (March 1994). "Assignment of the human ribosomal protein S25 gene (RPS25) to chromosome 11q23.3 by sequence analysis of the marker D11S456". Genomics. 20 (1): 142–3. doi:10.1006/geno.1994.1145. PMID 8020948.
• Miwa T, Sudo K, Nakamura Y, Imai T (July 1993). "Fifty sequenced-tagged sites on human chromosome 11". Genomics. 17 (1): 211–4. doi:10.1006/geno.1993.1304. PMID 8406452.
• Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, et al. (July 1996). "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry". European Journal of Biochemistry. 239 (1): 144–9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699.
• Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, et al. (May 1998). "A map of 75 human ribosomal protein genes". Genome Research. 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID 9582194.
• Kubota S, Copeland TD, Pomerantz RJ (February 1999). "Nuclear and nucleolar targeting of human ribosomal protein S25: common features shared with HIV-1 regulatory proteins". Oncogene. 18 (7): 1503–14. doi:10.1038/sj.onc.1202429. PMID 10050887. S2CID 25682899.
• Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (December 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
• Yoshihama M, Uechi T, Asakawa S, Kawasaki K, Kato S, Higa S, et al. (March 2002). "The human ribosomal protein genes: sequencing and comparative analysis of 73 genes". Genome Research. 12 (3): 379–90. doi:10.1101/gr.214202. PMC 155282. PMID 11875025.
• Lehner B, Sanderson CM (July 2004). "A protein interaction framework for human mRNA degradation". Genome Research. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Yu Y, Ji H, Doudna JA, Leary JA (June 2005). "Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes". Protein Science. 14 (6): 1438–46. doi:10.1110/ps.041293005. PMC 2253395. PMID 15883184.
• Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, et al. (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.