Jaswant Singh–Bhattacharji stain

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Jaswant Singh–Bhattacharji stain, commonly referred to as JSB stain, is a rapid staining method for detection of malaria.[1][2] It is useful for the diagnosis of malaria in thick smear samples of blood.[3] The JSB stain is commonly used throughout India, but rarely used in other countries.[4]

Composition

The JSB stain consists of two solutions which are used in sequence to stain various parts of the sample. The first solution consists of methylene blue, potassium dichromate, and sulfuric acid diluted in water. This solution is heated for several hours to oxidize the methylene blue. The second solution is eosin dissolved in water.[5]

See also

References

  1. ^ Singh, Jaswant; Bhattacharji, S; WHO Expert Committee on Malaria; WHO Malaria Conference in Equatorial Africa (1950 : Kampala, Uganda) (1950). "Rapid staining method of blood smears / Jaswant Singh, Bhattacharji". Geneva : World Health Organization. {{cite journal}}: Cite journal requires |journal= (help)CS1 maint: numeric names: authors list (link)
  2. ^ Manwell, RD; Feigelson, P (June 1948). "A modified method of preparing the J. S. B. stain". The Journal of Laboratory and Clinical Medicine. 33 (6): 777–82. PMID 18865757.
  3. ^ Annam, Vamseedhar; Mohan, ChakkiralaNalini; Mrinalini, VazhayilRamunny (2013). "Rapid detection of malaria parasite by toluidine blue method: A new staining method". Journal of Global Infectious Diseases. 5 (4): 153–5. doi:10.4103/0974-777X.122008. PMC 3958985. PMID 24672177.
  4. ^ Malaria Microscopy Quality Assurance Manual. World Health Organization. 2009. p. 29. ISBN 9789290614227.
  5. ^ US Dept. of the Army (1951). Methods for Medical Laboratory Technicians. US Government Printing Office. pp. 610–611.
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