|Source||Humanized (from mouse)|
|Drug class||Antibody-drug conjugate|
|Main uses||B-cell precursor acute lymphoblastic leukemia (ALL)|
|Side effects||Low platelets, low white blood cells, infection, low red blood cells, tiredness, bleeding, fever, nausea, headache, liver problems|
|Protein binding||97% (cytotoxic agent)|
|Elimination half-life||12.3 days|
|Chemical and physical data|
|Molar mass||146634.36 g·mol−1|
Inotuzumab ozogamicin, sold under the brand name Besponsa, is a medication used to treat B-cell precursor acute lymphoblastic leukemia (ALL). It is used when the cancer cells are CD22 positive and other treatments have failed. It is given by gradual injection into a vein.
Common side effects include low platelets, low white blood cells, infection, low red blood cells, tiredness, bleeding, fever, nausea, headache, and liver problems. Liver problems may include hepatic veno-occlusive disease. Other side effects may include QT prolongation and infertility. Use in pregnancy may harm the baby. It is a monoclonal antibody linked to N-acetyl-γ-calicheamicin. The antibody attaches to cells that express CD22 which brings about their death.
Inotuzumab ozogamicin was approved for medical use in the United States and Europe in 2017. In the United Kingdom it costs the NHS about £8,000 per 1 mg dose as of 2021. This amount in the United States costs about 24,000 USD.
It is administered by intravenous infusion in a doctor's office or clinic.
The most common serious side effects are infections (23%), loss of neutrophils with fever (11%), hemorrhage (5%), stomach pain (3%), fever (3%), VOD (2%), and tiredness (2%). More than 20% of people had the following: loss of platelets (51%), loss of neutrophils (49%), infections (48%), anemia (36%), leukopenia (35%), tiredness (35%), hemorrhage (33%), fever (32%), nausea (31%), headache (28%), loss of neutrophils with fever (26%), elevated transaminases (26%), stomach pain (23%), and jaundice (21%). Between 10% and 20% of people also had loss of appetite, vomiting, diarrhea, mouth sores, constipation, chills, and injection site reactions.
It carries an FDA black box warning concerning the risk of liver toxicity, in particular hepatic veno-occlusive disease (VOD), which has been fatal in some people. The risk of this is higher in people who take the drug before having hematopoietic stem cell transplantation (HSCT) and more people die who have HSCT following treatment with this drug, than people who have HSCT taking other chemotherapies. The risk gets higher as more rounds of treatment with inotuzumab ozogamicin are administered.
Pregnancy and breastfeeding
In studies in pregnant animals, the drug caused harm to the fetus at doses less than those used clinically, and so the drug has not been tested in pregnant women. Pregnant women should not take inotuzumab ozogamicin and must not become pregnant while taking it. It is unknown if the drug or its metabolites are secreted in breast milk, but women should not breastfeed while taking it, and should wait two months after the last dose to start breastfeeding.
The antibody component of inotuzumab ozogamicin binds to CD22 receptors, which are expressed mostly on B cells. The whole conjugate is then drawn into the cell, where the ozogamicin is cleaved from the antibody by the acidic environment of the lysosome. The ozogamicin eventually travels to the nucleus where it breaks up DNA, causing the cell to die.
Inotuzumab ozogamicin consists of the humanized monoclonal antibody inotuzumab (against CD22), linked to a cytotoxic agent from the class of calicheamicins called ozogamicin. Ozogamicin is N-acetyl-gamma-calicheamicin dimethylhydrazide. It includes the same linker, called "AcBut", and toxin, as gemtuzumab ozogamicin, which arose from the same collaboration. The linker is a carbonyl-containing carboxylic acid. The antibody, originally called G5/44, was created by grafting the complementarity-determining regions and some framework residues from the murine anti-CD22 mAb m5/44, onto human acceptor frameworks.
Celltech and Wyeth entered into a collaboration in 1991 to develop antibody-drug conjugates. It was discovered by scientists collaborating at Celltech and Wyeth, and it was developed by Pfizer which had acquired Wyeth.
The humanized antibody portion was generated at Celltech and the DNA encoding it was transfected into CHO cells, which were sent to Wyeth, where chemists expressed and purified the antibodies and conjugated them with the linker to the cytotoxin; the work was published in 2004. Celltech was acquired by UCB in 2004 and Wyeth was acquired by Pfizer in 2009.
In May 2013, a phase III trial in patients with relapsed or refractory CD22+ aggressive non-Hodgkin lymphoma (NHL) who were not candidates for intensive high-dose chemotherapy was terminated for futility.
In 2017, inotuzumab ozogamicin was approved by the European Commission and the FDA for the treatment of adults with relapsed or refractory CD22-positive B-cell precursor acute lymphoblastic leukemia (ALL) in 2017 under the trade name Besponsa (Pfizer/Wyeth).
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