Hippurate hydrolase

hippurate hydrolase
Identifiers
EC no.	3.5.1.32
CAS no.	37278-43-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a hippurate hydrolase (EC 3.5.1.32) is an enzyme that catalyzes the chemical reaction

hippurate + H₂O $\rightleftharpoons$ benzoate + glycine

Thus, the two substrates of this enzyme are hippurate and H₂O, whereas its two products are benzoate and glycine.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-benzoylamino-acid amidohydrolase. This enzyme participates in phenylalanine metabolism.

Hippurate hydrolysis test is used in the presumptive identification of Gardnerella vaginalis, Campylobacter jejuni, Listeria monocytogenes and group B streptococci by detecting the ability of the organism to hydrolyze hippurate.

References

• Tsoi E, Akmal S, Geerts L, Jeffery B, Nicolaides KH (2006). "Sonographic measurement of cervical length and fetal fibronectin testing in threatened preterm labor". Ultrasound Obstet. Gynecol. 27 (4): 368–72. doi:10.1002/uog.2723. PMID 16526097.
• Tsoi E, Akmal S, Geerts L, Jeffery B, Nicolaides KH (2006). "Sonographic measurement of cervical length and fetal fibronectin testing in threatened preterm labor". Ultrasound Obstet. Gynecol. 27 (4): 368–72. doi:10.1002/uog.2723. PMID 16526097.
• Rohrmann K, Niemann R, Buddecke E (1985). "Two N-acetylgalactosaminyltransferase are involved in the biosynthesis of chondroitin sulfate". Eur. J. Biochem. 148 (3): 463–9. doi:10.1111/j.1432-1033.1985.tb08862.x. PMID 3922754.