File:PMC2713404 ppat.1000536.g001.png

English⧼Colon⧽ ppat-1000536-g001: Immunohistochemical localization of Marburg virus antigens in Roussetus aegypticus tissues.In the liver, viral antigens were distributed in and around hepatocytes in a dense (A) or loose (B) perimembranous pattern. Rarely, entire hepatocytes were involved (C). These infected foci were characteristically sparse and were often associated with small collections of mononuclear inflammatory cells and hepatocyte necrosis (D and E), although infected cells could also be identified without conspicuous inflammatory infiltrates. Only rare viral antigens were seen in a few mononuclear cells of the spleen of 1 bat (F). Immunoalkaline phosphatase with napthol fast-red and hematoxylin counterstain (A–C, E, F), and hematoxylin and eosin (D); original magnifications ×100 (A, B, D, E) and ×258 (C, F).