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Trade namesBetasept, ChloraPrep, Chlorostat, others
Other namesChlorhexidine gluconate (CHG), chlorhexidine acetate, 1,6-bis(4-chloro-phenylbiguanido)hexane
  • N,N′′′′1,6-Hexanediylbis[N′-(4-chlorophenyl)(imidodicarbonimidic diamide)]
Clinical data
Drug classDisinfectant, antiseptic
Main usesSkin disinfection before surgery, sterilize surgical instruments[1]
Side effectsSkin irritation, teeth discoloration, allergic reactions[2]
  • US: B (No risk in non-human studies)and C[3]
Routes of
Topical, mouth wash
Defined daily dose30 mg (by mouth) periodontal treatment[4]
30 mg (by mouth) local oral infection-treatment[5]
External links
AHFS/Drugs.comTopical: Monograph
Mouth: Monograph
License data
Legal status
  • US: OTC / Rx-only
Chemical and physical data
Molar mass505.45 g·mol−1
3D model (JSmol)
Melting point134 to 136 °C (273 to 277 °F)
Solubility in water0.8 mg/mL (20 °C)
  • Clc1ccc(NC(=N)NC(=N)NCCCCCCNC(=N)NC(=N)Nc2ccc(Cl)cc2)cc1
  • InChI=1S/C22H30Cl2N10/c23-15-5-9-17(10-6-15)31-21(27)33-19(25)29-13-3-1-2-4-14-30-20(26)34-22(28)32-18-11-7-16(24)8-12-18/h5-12H,1-4,13-14H2,(H5,25,27,29,31,33)(H5,26,28,30,32,34) checkY

Chlorhexidine is a disinfectant and antiseptic that is used for skin disinfection before surgery and to sterilize surgical instruments.[1] It may be used both to disinfect the skin of the patient and the hands of the healthcare providers.[2] It is also used for cleaning wounds, preventing dental plaque, treating yeast infections of the mouth, and to keep urinary catheters from blocking.[2] It is used as a liquid or powder.[1][2]

Side effects may include skin irritation, teeth discoloration, and allergic reactions.[2] It may cause eye problems if direct contact occurs.[3] Use in pregnancy appears to be safe.[6] Chlorhexidine may come mixed in alcohol, water, or surfactant solution.[2] It is effective against a range of microorganisms, but does not inactivate spores.[1]

Chlorhexidine came into medical use in the 1950s.[7] It is on the World Health Organization's List of Essential Medicines.[8] Chlorhexidine is available over the counter (OTC) in the United States.[3] The wholesale cost in the developing world is about US$2.20–4.10 per liter of 5% solution.[9] In the United Kingdom this amount costs the NHS about £4.80.[2] In 2017, it was the 286th most commonly prescribed medication in the United States, with more than one million prescriptions.[10][11]


Chlorhexidine is used in disinfectants (disinfection of the skin and hands), cosmetics (additive to creams, toothpaste, deodorants, and antiperspirants), and pharmaceutical products (preservative in eye drops, active substance in wound dressings and antiseptic mouthwashes).[12] A 2019 Cochrane review concluded that based on very low certainty evidence in those who are critical ill "it is not clear whether bathing with chlorhexidine reduces hospital‐acquired infections, mortality, or length of stay in the ICU, or whether the use of chlorhexidine results in more skin reactions."[13]

In endodontics, chlorhexidine is used for root canal irrigation and as an intracanal dressing,[14][15][16] but has been replaced by the use of sodium hypochlorite bleach in much of the developed world.


There is tentative evidence that it is more effective than povidone-iodine.[17]

CHG is active against Gram-positive and Gram-negative organisms, facultative anaerobes, aerobes, and yeasts.[18] It is particularly effective against Gram-positive bacteria (in concentrations ≥ 1 μg/l). Significantly higher concentrations (10 to more than 73 μg/ml) are required for Gram-negative bacteria and fungi. Chlorhexidine is ineffective against polioviruses and adenoviruses. The effectiveness against herpes viruses has not yet been established unequivocally.[19]

Chlorhexidine, like other cation-active compounds, remains on the skin. It is frequently combined with alcohols (ethanol and isopropyl alcohol).


Perichlor brand 0.12% chlorhexidine gluconate solution

Use of a CHG-based mouthwash in combination with normal tooth care can help reduce the build-up of plaque and improve mild gingivitis.[20] There is not enough evidence to determine the effect in moderate to severe gingivitis.[20] About 20 mL twice a day of concentrations of 0.1% to 0.2% is recommended for mouth-rinse solutions with a duration of at least 30 seconds.[20] Such mouthwash also has a number of adverse effects including damage to the mouth lining, tooth discoloration, tartar build-up, and impaired taste.[20] Extrinsic tooth staining occurs when chlorhexidine rinse has been used for 4 weeks or longer.[20]

Mouthwashes containing chlorhexidine which stain teeth less than the classic solution have been developed, many of which contain chelated zinc.[21][22][23]

Using chlorhexidine as a supplement to everyday mechanical oral hygiene procedures for 4 to 6 weeks and 6 months leads to a moderate reduction in gingivitis compared to placebo, control or mechanical oral hygiene alone.[20]

Chlorhexidine is a cation which interacts with anionic components of toothpaste, such as sodium lauryl sulfate and sodium monofluorophosphate, and forms salts of low solubility and antibacterial activity. Hence, to enhance the antiplaque effect of chlorhexidine, "it seems best that the interval between toothbrushing and rinsing with CHX [chlorhexidine] be more than 30 minutes, cautiously close to 2 hours after brushing.".[24]


Nepal was the first country in the world to use chlorhexidine to treat the umbilical cord of newborn babies, and received a USAID Pioneers Prize for reducing the neonatal death rate.[25] Chlorhexidine is very effective for poor countries like Nepal and its use is growing in the world for treating the umbilical cord. A 2015 Cochrane review has yielded high-quality evidence that within the community setting, chlorhexidine skin or cord care can reduce the incidence of omphalitis (inflammation of the umbilical cord) by 50% and also neonatal mortality by 12%.[26] Chlorhexidine gluconate is used as a skin cleanser for surgical scrubs, a cleanser for skin wounds, for preoperative skin preparation and germicidal hand rinses.[18] Chlorhexidine eye drops have been used as a treatment for eyes affected by Acanthamoeba keratitis.[27]


The defined daily dose is 30 mg by mouth for dental treatment and mouth infections.[4][5]

Side effects

Half an hour after chlorhexidine skin prick individual complained of febrile sensation, facial swelling, itchy skin rash.

CHG is ototoxic; if put into an ear canal which has a ruptured eardrum, it can lead to deafness.[28]

CHG does not meet current European specifications for a hand disinfectant. Under the test conditions of the European Standard EN 1499, no significant difference in the efficacy was found between a 4% solution of chlorhexidine digluconate and soap.[19] In the U.S., between 2007 and 2009, Hunter Holmes McGuire Veterans Administration Medical Center conducted a cluster-randomized trial and concluded that daily bathing of patients in intensive care units with washcloths saturated with chlorhexidine gluconate reduced the risk of hospital-acquired infections.[29]

Whether prolonged exposure over many years may have carcinogenic potential is still not clear. The Federal Drug Administration (FDA) in the USA recommendation is to limit the use of a chlorhexidine gluconate mouthwash to a maximum of six months.[30]

Once digested CHG is poorly absorbed into gastrointestinal tract.[31][32] If aspirated into the lungs at high enough concentration, as reported in one case, it can be fatal due to the high risk of acute respiratory distress syndrome.[32][33]

Mechanism of action

At physiologic pH, chlorhexidine salts dissociate and release the positively charged chlorhexidine cation. The bactericidal effect is a result of the binding of this cationic molecule to negatively charged bacterial cell walls. At low concentrations of chlorhexidine, this results in a bacteriostatic effect; at high concentrations, membrane disruption results in cell death.[18]


It is a cationic polybiguanide (bisbiguanide).[34] It is used primarily as its salts (e.g., the dihydrochloride, diacetate, and digluconate).[citation needed]


Chlorhexidine is deactivated by forming insoluble salts with anionic compounds, including the anionic surfactants commonly used as detergents in toothpastes and mouthwashes, anionic thickeners such as carbomer, and anionic emulsifiers such as acrylates/C10-30 alkyl acrylate crosspolymer, among many others. For this reason, chlorhexidine mouth rinses should be used at least 30 minutes after other dental products.[35] For best effectiveness, food, drink, smoking, and mouth rinses should be avoided for at least one hour after use. Many topical skin products, cleansers, and hand sanitizers should also be avoided to prevent deactivation when chlorhexidine (as a topical by itself or as a residue from a cleanser) is meant to remain on the skin.[citation needed]


The structure is based on two molecules of proguanil, linked with a hexamethylenediamine spacer.

Two routes for chlorhexidine synthesis:[36] U.S. Patent 2,684,924 (1954 to I.C.I.). The compounds designated (...)2 are substituted hexanes.


Chlorhexidine topical is sold as Betasept, Biopatch, Calgon Vesta, ChloraPrep One-Step, Dyna-Hex, Hibiclens, Hibistat Towelette, Scrub Care Exidine, Spectrum-4 among others.[37]

Chlorhexidine gluconate mouth rinse is sold as Paroex, Peridex, PerioChip, Periogard, Dentohexin among others.[38]

Hexoralettene N contains benzocaine, menthol and chlorhexidine hydrochloride. It is used as oral antiseptic candies.[citation needed]


The name "Chlorhexidine" breaks down as chlor(o) + hex(ane) + id(e) + (am)ine), is a cationic polybiguanide. It is used primarily as its gluconate salt.

Veterinary medicine

In animals, chlorhexidine is used for topical disinfection of wounds,[39] and to manage skin infections.[40] Chlorhexidine-based disinfectant products are used within the dairy farming industry.[41]

Post-surgical respiratory problems have been associated with the use of chlorhexidine products in cats.[42]

Society and culture


The wholesale cost in the developing world is about US$2.20–4.10 per liter of 5% solution.[9] In the United Kingdom this amount costs the NHS about £4.80.[2] In 2017, it was the 286th most commonly prescribed medication in the United States, with more than one million prescriptions.[10][11]

See also


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  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 British national formulary : BNF 69 (69 ed.). British Medical Association. 2015. pp. 568, 791, 839. ISBN 9780857111562.
  3. 3.0 3.1 3.2 "Chlorhexidine Gluconate topical". The American Society of Health-System Pharmacists. Archived from the original on 13 January 2017. Retrieved 8 January 2017.
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  6. Briggs, Gerald G.; Freeman, Roger K.; Yaffe, Sumner J. (2011). Drugs in Pregnancy and Lactation: A Reference Guide to Fetal and Neonatal Risk. Lippincott Williams & Wilkins. p. 252. ISBN 9781608317080. Archived from the original on 2017-01-13.
  7. Schmalz, Gottfried; Bindslev, Dorthe Arenholt (2008). Biocompatibility of Dental Materials. Springer Science & Business Media. p. 351. ISBN 9783540777823. Archived from the original on 2017-01-13.
  8. World Health Organization (2019). World Health Organization model list of essential medicines: 21st list 2019. Geneva: World Health Organization. hdl:10665/325771. WHO/MVP/EMP/IAU/2019.06. License: CC BY-NC-SA 3.0 IGO.
  9. 9.0 9.1 "Chlorhexidine Gluconate". International Drug Price Indicator Guide. Archived from the original on 10 May 2017. Retrieved 8 December 2016.
  10. 10.0 10.1 "The Top 300 of 2020". ClinCalc. Archived from the original on 12 February 2021. Retrieved 11 April 2020.
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  12. Thomas Güthner; et al. (2007), "Guanidine and Derivatives", Ullman's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, p. 13
  13. Lewis, Sharon R.; Schofield-Robinson, Oliver J.; Rhodes, Sarah; Smith, Andrew F. (30 August 2019). "Chlorhexidine bathing of the critically ill for the prevention of hospital‐acquired infection". Cochrane Database of Systematic Reviews. 8: CD012248. doi:10.1002/14651858.CD012248.pub2. PMC 6718196. PMID 31476022.
  14. Raab D: Preparation of contaminated root canal systems – the importance of antimicrobial irrigants. DENTAL INC 2008: July / August 34–36.
  15. Raab D, Ma A: Preparation of contaminated root canal systems – the importance of antimicrobial irrigants. 经感染的根管系统的修复 – 化学冲洗对根管治疗的重要性DENTAL INC Chinese Edition 2008: August 18–20.
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  18. 18.0 18.1 18.2 Leikin, Jerrold B.; Paloucek, Frank P., eds. (2008), "Chlorhexidine Gluconate", Poisoning and Toxicology Handbook (4th ed.), Informa, pp. 183–84
  19. 19.0 19.1 Hans-P. Harke (2007), "Disinfectants", Ullman's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 10–11
  20. 20.0 20.1 20.2 20.3 20.4 20.5 James P, Worthington HV, Parnell C, Harding M, Lamont T, Cheung A, Whelton H, Riley P (2017). "Chlorhexidine mouthrinse as an adjunctive treatment for gingival health". Cochrane Database Syst Rev. 3: CD008676. doi:10.1002/14651858.CD008676.pub2. PMC 6464488. PMID 28362061. Archived from the original on 2021-08-28. Retrieved 2019-05-29.
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  34. Tanzer JM, Slee AM, Kamay BA (1977). "Structural requirements of guanide, biguanide, and bisbiguanide agents for antiplaque activity". Antimicrob. Agents Chemother. 12 (6): 721–9. doi:10.1128/aac.12.6.721. PMC 430011. PMID 931371.
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External links

External sites: