ALDH2

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ALDH2
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesALDH2, ALDH-E2, ALDHI, ALDM, aldehyde dehydrogenase 2 family (mitochondrial), aldehyde dehydrogenase 2 family member
External IDsOMIM: 100650 MGI: 99600 HomoloGene: 55480 GeneCards: ALDH2
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001204889
NM_000690

NM_009656
NM_001308450

RefSeq (protein)

NP_000681
NP_001191818

NP_001295379
NP_033786

Location (UCSC)Chr 12: 111.77 – 111.82 MbChr 5: 121.7 – 121.73 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Aldehyde dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the ALDH2 gene located on chromosome 12.[5][6] ALDH2 belongs to the aldehyde dehydrogenase family of enzymes. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH2 has a low Km for acetaldehyde, and is localized in mitochondrial matrix. The other liver isozyme, ALDH1, localizes to the cytosol.[7]

Most White people have both major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not a functional mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Whites could be related to this absence of a catalytically active form of ALDH2. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer.[8]

Gene

The ALDH2 gene is about 44 kbp in length and contains at least 13 exons which encode 517 amino acid residues. Except for the signal NH2-terminal peptide, which is absent in the mature enzyme, the amino acid sequence deduced from the exons coincided with the reported primary structure of human liver ALDH2. Several introns contain Alu repetitive sequences. A TATA-like sequence (TTATAAAA) and a CAAT-like sequence (GTCATCAT) are located 473 and 515 bp, respectively, upstream from the translation initiation codon.[9] In mice the official full name of the gene is aldehyde dehydrogenase 2, mitochondrial, and it is positioned on chromosome 5. It has 12 exons and the coding sequence is 1,560 base pairs.[10]

Enzyme structure

The enzyme encoded by the human ALDH2 gene is a tetrameric enzyme that contains three domains; two dinucleotide-binding domains and a three-stranded beta-sheet domain. The active site of ALDH2 is divided into two halves by the nicotinamide ring of NAD+. Adjacent to the A-side (Pro-R) of the nicotinamide ring is a cluster of three cysteines (Cys301, Cys302 and Cys303) and adjacent to the B-side (Pro-S) are Thr244, Glu268, Glu476 and an ordered water molecule bound to Thr244 and Glu476.[11] Although there is a recognizable Rossmann fold, the coenzyme-binding region of ALDH2 binds NAD+ in a manner not seen in other NAD+-binding enzymes. The positions of the residues near the nicotinamide ring of NAD+ suggest a chemical mechanism whereby Glu268 functions as a general base through a bound water molecule. The sidechain amide nitrogen of Asn169 and the peptide nitrogen of Cys302 are in position to stabilize the oxyanion present in the tetrahedral transition state prior to hydride transfer. The functional importance of residue Glu487 now appears to be due to indirect interactions of this residue with the substrate-binding site via Arg264 and Arg475.[12]

Function

Mitochondrial aldehyde dehydrogenase belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Human ALDH2 is especially efficient on acetaldehyde compared to ALDH1.[13]

Ethanol metabolism in humans
Ethanol metabolism in humans

Additionally, ALDH2 functions as a protector against oxidative stress.[14]

Genetic variation

SNP: ALDH2*2
Name(s)g.42421G>A, Glu504Lys
GeneALDH2
Chromosome12
RegionExon
External databases
EnsemblHuman SNPView
dbSNP671
HapMap671
SNPedia671

The inactivating ALDH2*2 mutation is "the most common single point mutation in humans".[15] This mutation is found in very few White people, but about 50% of East Asians are heterozygous for this mutation. The ALDH2*2 allele encodes lysine instead of glutamic acid at amino acid 487,[16] distorting the NAD+ binding site.[17][18] ALDH2 assembles and functions as a tetramer and requires all four of its components to be active in order to metabolize acetaldehyde. People heterozygous for ALDH2*2 have only 10% to 45% enzyme activity, while those homozygous for ALDH2*2 have as little as 1% to 5% remaining activity.[19]

The lack of ALDH2 activity has a number of consequences, detailed in section § Inhibition and genetic deficiency below.

Distribution

In the overall Japanese population, about 57% of individuals are homozygous for the normal allele, 40% are heterozygous for the ALDH2*2 allele, and 3% are homozygous for the ALDH2*2 allele.[16]

Clinical significance

Inhibition and genetic deficiency

Alcohol metabolism

The best-known consequence of ALDH2 dysfunction is in relation to the consumption of ethanol. People heterozygous or homozygous for the ALDH2*2 metabolize ethanol to acetaldehyde normally but metabolize acetaldehyde poorly. As a result, they accumulate increased levels of acetaldehyde after consumption of alcoholic beverages. Effects include facial flushing (i.e. the "Alcohol flush reaction"), urticaria, systemic dermatitis, and alcohol-induced respiratory reactions such as rhinitis and the exacerbation of asthma bronchoconstriction.[20] The cited allergic reaction-like symptoms: (a) do not appear due to classical IgE or T cell-related allergen-induced reactions but rather the actions of acetaldehyde in stimulating the release of histamine, a probable mediating cause of these symptoms; (b) typically occur within 30–60 minutes of ingesting alcoholic beverages; and (c) occur in other Asian as well as non-Asian individuals that are either seriously defective in metabolizing ingested ethanol past acetaldehyde to acetic acid or, alternatively, that metabolize ethanol too rapidly for ALDH2 processing.[20][21]

People with a genetic ALDH2*2 deficiency have historically had a lower likelihood of developing alcoholism, both from stronger adverse effects and a possible reduction of dopamine release.[22] However, this effect is not absolute: during the 1980s, there has been a steady increase in the number of Japanese alcoholics who carry the ALDH2*2 mutation. A strong social pressure to drink have overcome this genetic barrier to alcoholism.[23] Disulfiram, which inhibits ALDH2 and causes a similar effect, has been used as an alcohol-quitting aid.[22]

Various conditions

More recently, ALDH2 has been implicated in a number of pathways beyond alcohol metabolism. ALDH2 dysfunction is supposedly associated with a variety of human diseases including diabetes, neurodegenerative diseases, cardiovascular diseases and stroke, cancer, Fanconi anemia, pain, osteoporosis, and the process of aging.[15] The inactivating ALDH2 rs671 polymorphism, present in up to 8% of the global population and in up to 50% of the East Asian population, is associated with increased risk of cardiovascular conditions such as coronary artery disease, alcohol-induced cardiac dysfunction, pulmonary arterial hypertension, heart failure and drug-induced cardiotoxicity.[24]

Alzheimer's disease

A case-control study in a Japanese population showed that deficiency of ALDH2 activity influences the risk for late-onset Alzheimer's disease.[14] The ALDH2 knockout mice display age-related memory deficits in various tasks, as well as endothelial dysfunction, brain atrophy, and other Alzheimer's disease-associated pathologies, including marked increases in lipid peroxidation products, amyloid-beta, p-tau and activated caspases. These behavioral and biochemical Alzheimer's disease-like deficits were efficiently ameliorated when these mice were treated with isotope-reinforced lipids (deuterated polyunsaturated fatty acids).[25]

Activation

An activator of ALDH2 enzymatic activity, Alda-1 (N-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamide), has been shown to reduce ischemia-induced cardiac damage caused by myocardial infarction.[26]

Interactions

ALDH2 has been shown to interact with GroEL.[27]

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000111275 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029455 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
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  6. ^ Hsu LC, Tani K, Fujiyoshi T, Kurachi K, Yoshida A (Jun 1985). "Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2". Proceedings of the National Academy of Sciences of the United States of America. 82 (11): 3771–5. Bibcode:1985PNAS...82.3771H. doi:10.1073/pnas.82.11.3771. PMC 397869. PMID 2987944.
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  10. ^ van Warmerdam T. "YourBioHelper.com". Archived from the original on 2021-12-19.
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  17. ^ Larson HN, Weiner H, Hurley TD (August 2005). "Disruption of the coenzyme binding site and dimer interface revealed in the crystal structure of mitochondrial aldehyde dehydrogenase "Asian" variant". The Journal of Biological Chemistry. 280 (34): 30550–6. doi:10.1074/jbc.M502345200. PMC 1262676. PMID 15983043.
  18. ^ Chang HY, Mitchell A (May 2014). "Dionysian Mysteries The Aldehyde Dehydrogenase (aldh) Family". InterPro Protein Focus.
  19. ^ Ma C, Yu B, Zhang W, Wang W, Zhang L, Zeng Q (11 September 2017). "Associations between aldehyde dehydrogenase 2 (ALDH2) rs671 genetic polymorphisms, lifestyles and hypertension risk in Chinese Han people". Scientific Reports. 7 (1): 11136. Bibcode:2017NatSR...711136M. doi:10.1038/s41598-017-11071-w. PMC 5593832. PMID 28894224.
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  22. ^ a b Chen CH, Ferreira JC, Gross ER, Mochly-Rosen D (2014). "Targeting Aldehyde Dehydrogenase 2: New Therapeutic Opportunities". Physiological Reviews. 94 (1): 1–34. doi:10.1152/physrev.00017.2013. PMC 3929114. PMID 24382882.
  23. ^ Higuchi S, Matsushita S, Imazeki H, Kinoshita T, Takagi S, Kono H (March 1994). "Aldehyde dehydrogenase genotypes in Japanese alcoholics". Lancet. 343 (8899): 741–2. doi:10.1016/S0140-6736(94)91629-2. PMID 7907720. S2CID 41404745.
  24. ^ Zhang J, Guo Y, Zhao X, Pang J, Pan C, Wang J, Wei S, Yu X, Zhang C, Chen Y, Yin H, Xu F (2023). "The role of aldehyde dehydrogenase 2 in cardiovascular disease". Nature Reviews. Cardiology. 20 (7): 495–509. doi:10.1038/s41569-023-00839-5. PMID 36781974. S2CID 256845243.
  25. ^ Elharram A, Czegledy NM, Golod M, Milne GL, Pollock E, Bennett BM, Shchepinov MS (December 2017). "Deuterium-reinforced polyunsaturated fatty acids improve cognition in a mouse model of sporadic Alzheimer's disease". The FEBS Journal. 284 (23): 4083–4095. doi:10.1111/febs.14291. PMC 5716852. PMID 29024570.
  26. ^ Chen CH, Budas GR, Churchill EN, Disatnik MH, Hurley TD, Mochly-Rosen D (Sep 2008). "Activation of aldehyde dehydrogenase-2 reduces ischemic damage to the heart". Science. 321 (5895): 1493–5. Bibcode:2008Sci...321.1493C. doi:10.1126/science.1158554. PMC 2741612. PMID 18787169.
  27. ^ Lee KH, Kim HS, Jeong HS, Lee YS (Oct 2002). "Chaperonin GroESL mediates the protein folding of human liver mitochondrial aldehyde dehydrogenase in Escherichia coli". Biochemical and Biophysical Research Communications. 298 (2): 216–24. doi:10.1016/S0006-291X(02)02423-3. PMID 12387818.

Further reading

External links