4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
Jump to navigation
Jump to search
| 4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.1.182 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase (EC 3.2.1.182, DIMBOAGlc hydrolase, DIMBOA glucosidase) is an enzyme with systematic name (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside beta-D-glucosidase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
- (1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
- (2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
The enzyme from Triticum aestivum (wheat) has a higher affinity for DIMBOA glucoside than DIBOA glucoside.
References
- ^ Sue M, Ishihara A, Iwamura H (February 2000). "Purification and characterization of a hydroxamic acid glucoside beta-glucosidase from wheat (Triticum aestivum L.) seedlings". Planta. 210 (3): 432–8. doi:10.1007/s004250050029. PMID 10750901. S2CID 6266316.
- ^ Sue M, Ishihara A, Iwamura H (June 2000). "Purification and characterization of a beta-glucosidase from rye (Secale cereale L.) seedlings". Plant Science. 155 (1): 67–74. doi:10.1016/s0168-9452(00)00204-1. PMID 10773341.
- ^ Czjzek M, Cicek M, Zamboni V, Bevan DR, Henrissat B, Esen A (December 2000). "The mechanism of substrate (aglycone) specificity in beta -glucosidases is revealed by crystal structures of mutant maize beta -glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes". Proceedings of the National Academy of Sciences of the United States of America. 97 (25): 13555–60. Bibcode:2000PNAS...9713555C. doi:10.1073/pnas.97.25.13555. PMC 17614. PMID 11106394.
- ^ Nikus J, Esen A, Jonsson LM (2003). "Cloning of a plastidic rye (Secale cereale) β-glucosidase cDNA and its expression in Escherichia coli". Physiol. Plant. 118 (3): 337–348. doi:10.1034/j.1399-3054.2003.00118.x.
- ^ Sue M, Yamazaki K, Yajima S, Nomura T, Matsukawa T, Iwamura H, Miyamoto T (August 2006). "Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye". Plant Physiology. 141 (4): 1237–47. doi:10.1104/pp.106.077693. PMC 1533919. PMID 16751439.
- ^ Sue M, Nakamura C, Miyamoto T, Yajima S (February 2011). "Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae". Plant Science. 180 (2): 268–75. doi:10.1016/j.plantsci.2010.09.001. PMID 21421370.
External links
- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl+glucoside+beta-D-glucosidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
